Purpose The aim of this study was to assess splenic volume and to correlate unidimensional measurements with reference volumetric changes in chemotherapy-treated patients with colorectal cancer (CRC) liver metastases. correlation (< 0.05). The mean reference splenic volume increased after 6 months of chemotherapy compared to baseline (326 vs. 278 mL). Splenic volume changes showed the highest correlation with changes in (< 0.001), 104615-18-1 IC50 then (< 0.001), but were not significantly correlated with changes in (= 0.055). Conclusions Our results show the potential utility of measuring changes in splenic width to predict clinically significant changes in splenic volume in chemotherapy-treated patients with CRC liver metastases. 0.52, also provide estimates of splenic volumes [1, 3, 8, 10, 11]. Both of these methods presume an upper limit of 314.5 mL 104615-18-1 IC50 for normal splenic volume [7, 8, 11, 12]. A method to assess splenic enlargement that is both accurate and practical would be helpful for patients with CRC liver metastases as those who develop splenomegaly as a result of chemotherapy are at risk for complications . Even though radiological studies cited above have emphasized accuracy of splenic volumetry to evaluate for splenomegaly, the existing methods are cumbersome in clinical practice [7, 8, 11, 12]. Our hypothesis is usually that a unidimensional switch can accurately reflect a splenic volumetric switch following chemotherapy. To this end, the purpose of this study was to assess splenic volumes in CRC patients with liver metastases undergoing chemotherapy and to correlate unidimensional measurements with reference volumetric changes. Methods and materials Patient selection Our hospital's Institutional Review Table approved this study via a waiver of the Health Insurance Portability and PRPF38A Accountability Take action. In this retrospective study, 40 consecutive patients were selected from a cohort of 384 patients included in a previously reported study of chemotherapy-related morbidity following major hepatectomy for CRC liver metastases . The 40 patients were treated with chemotherapy for 6 months prior to resection, with imaging performed at baseline (before chemotherapy) and after 6 months of chemotherapy (before resection). Chemotherapy regimens consisted of either FOLFOX (5-fluorouracil, leucovorin, and oxaliplatin) or FOLFIRI (5-fluorouracil, leucovorin, and irinotecan) . Splenic measurements were performed at baseline (0 months) and 6 months. Patient age, gender, and excess weight were collected prospectively and examined retrospectively. The median age of the study populace was 61 years (range 28C83 years) and 67% of patients were male. Both the chemotherapy and surgical management have been explained previously . Image analysis Contrast-enhanced CT images acquired during the portal venous phase were reviewed by a medical student, under supervision by an attending radiologist, on a PACS workstation (picture archiving and communication system, Centricity PACS 2.0, GE Healthcare, Milwaukee, WI, USA), and with a GE Advantage Workstation available for multiplanar reconstruction. Three unidimensional measurements of the spleen were undertakenwidth (was defined as the maximum length measured on transverse images, regardless of obliquity. Splenic was defined as the maximum perpendicular length around the transverse section at the splenic hilum where splenic vessels enter and leave the spleen. Splenic was measured as the maximal craniocaudal dimensions by one of two methods. If the slice positions were available for the transverse images on PACS, the difference between the first and last slice position was used to calculate ((((< 0.001) and (< 0.001) were better correlated than (< 0.05). 104615-18-1 IC50 Comparable results were also found at 6 months (Fig. 5). Ellipsoid volumes were similarly correlated with reference splenic volumes at baseline (< 0.001) and 6 months (< 0.001) after chemotherapy (Figs. 4, ?,5;5; Table 1). Fig. 4 Scatterplot of the regression analysis of unidimensional measurements and ellipsoid volume correlated with reference splenic volumes in 40 patients at baseline with the 95% confidence interval ((< 0.001) and (< 0.001). Changes in splenic volume showed higher correlation with than with EV (< 0.001), whereas splenic volume changes were not significantly correlated with (= 0.055) (Fig. 6). The linear regression equations for and are as follows: (< 0.001) and.