Supplementary MaterialsFigure 1source data 1: Parallel measurements of fluorescence levels by flow cytometry and of mRNA levels by pyrosequencing. assays between strains with low and high values of expression noise. Competitive fitness was measured by pyrosequencing and analyzed using the R script provided in Supplementary file 3. Data used to make Figure 4. elife-37272-fig4-data1.xlsx (16K) DOI:?10.7554/eLife.37272.022 Figure 5figure supplement 1source data 1: Single-cell measures of doubling time in four strains with different median levels and noise of?TDH3?expression. Doubling time was determined from time-lapse microscopy data.?These data were used to make Figure 5figure supplement 1. elife-37272-fig5-figsupp1-data1.xlsx (248K) DOI:?10.7554/eLife.37272.025 Figure 5figure supplement 1source data 2: Summary statistics for comparing the distributions of single-cell doubling time between genotypes with different expression noise levels. These data Vorinostat ic50 were used to make Figure Vorinostat ic50 5figure supplement Vorinostat ic50 1. elife-37272-fig5-figsupp1-data2.xlsx (101K) DOI:?10.7554/eLife.37272.026 Figure 6source data 1: Fitness data obtained by modeling the growth of cell populations with different levels of mean expression and expression noise. Data used to make Shape D and 6B and generated using the Matlab code provided in Supplementary document 5. elife-37272-fig6-data1.xlsx (36K) DOI:?10.7554/eLife.37272.030 Supplementary file 1: Datasets generated using R scripts obtainable in Supplementary file 3 and used to create Source Documents for figures. elife-37272-supp1.xlsx (939K) DOI:?10.7554/eLife.37272.031 Supplementary file 2: Set of oligonucleotides found in this research. elife-37272-supp2.xlsx (15K) DOI:?10.7554/eLife.37272.032 Supplementary file 3: R scripts for the analysis of pyrosequencing and flow cytometry data used to determine the median expression, expression noise and fitness associated with different alleles. elife-37272-supp3.r (290K) DOI:?10.7554/eLife.37272.033 Supplementary file 4: Zip folder containing all input files necessary to run the R scripts available in Supplementary file 3. elife-37272-supp4.zip (385K) DOI:?10.7554/eLife.37272.034 Supplementary file 5: Matlab code used to model the growth of cell populations with different levels of mean expression and expression noise. elife-37272-supp5.m (10K) DOI:?10.7554/eLife.37272.035 Source data 1: Expression and fitness data for the final set of 43 promoter alleles. Fluorescence levels and competitive fitness were measured by flow cytometry and analyzed using the R script provided in Supplementary file 3. Data used to make Figure 1D, Figure 2C and Figure 3. elife-37272-data1.xls (86K) DOI:?10.7554/eLife.37272.036 Transparent reporting form. elife-37272-transrepform.docx (247K) DOI:?10.7554/eLife.37272.037 Data Availability StatementFlow data Vorinostat ic50 (FCS files) used to quantify fluorescence levels produced by the 43 TDH3 promoter alleles are available in the FlowRepository (flowrepository.org) under experiment ID FR-FCM-ZY8Y. Raw bright field and fluorescence images, as well as bright field images where cell division events were annotated, are available on Zenodo (https://zenodo.org) with DOI 10.5281/zenodo.1327545. All other data are provided as source data and/or supplementary files with the manuscript. Vorinostat ic50 The BPES following datasets were generated: Fabien Duveau2018Time-lapse images of yeast cells with different levels of TDH3 median expression and expression noisehttps://dx.doi.org/10.5281/zenodo.1327545Publicly available at Zenodo (https://zenodo.org/). Fabien DuveauPatricia J Wittkopp2016Activity of 48 TDH3 promoter variations in YPDhttp://flowrepository.org/id/FR-FCM-ZY8YPublicly offered by FlowRepository (accession zero. FR-FCM-ZY8Y) Fabien DuveauPatricia J Wittkopp2016Effects of 20 TDH3 promoter alleles at HO and indigenous TDH3 locihttp://flowrepository.org/id/FR-FCM-ZYJXPublicly offered by FlowRepository (accession simply no. FR-FCM-ZYJX) Fabien DuveauPatricia J Wittkopp2016Fitness of 48 TDH3 promoter alleles in YPDhttp://flowrepository.org/id/FR-FCM-ZYJNPublicly offered by FlowRepository (accession simply no. FR-FCM-ZYJN) Abstract Gene manifestation noise can be an evolvable home of natural systems that identifies differences in manifestation among genetically similar cells in the same environment. Prior function shows that manifestation noise can be heritable and may be formed by selection, however the effect of variant in manifestation noise on organismal fitness has proven difficult to measure. Here, we quantify the fitness effects of altering expression noise for the gene in gene of encodes an isozyme of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) involved in.