Supplementary MaterialsAdditional file 1: Figure S1. of protein phosphorylation between MS patients and controls after in vitro stimulation. Fold change in the levels phosphorylated proteins induced by in vitro stimulation in each cell type in healthy controls and RRMS patients. Ideals represent the mean collapse modification of phosphorylation amounts and regular deviation for every combined group. (DOCX 15?kb) 12974_2018_1105_MOESM3_ESM.docx (15K) GUID:?68BEC7E0-5574-43EE-9C8C-90689443A546 Additional document 4: Desk S3. Assessment of degrees of phosphorylated protein between MS settings and individuals after in vitro excitement. Degrees of phosphorylated protein in each cell enter healthy RRMS and settings individuals. Ideals represent the mean fluorescence strength and regular deviation for every combined group. (DOCX 14?kb) 12974_2018_1105_MOESM4_ESM.docx Rabbit Polyclonal to MRPL12 (15K) GUID:?7645CEA6-BA27-46D2-A6C6-5912D61804D7 Extra file 5: Desk S4. Assessment of degrees of p38MAPK, Erk1/2, STAT1, and STAT6 between MS settings and individuals. Degrees of chosen proteins in each cell enter healthful settings and RRMS individuals. Values represent the mean fluorescence intensity and standard deviation for each group. (DOCX 13?kb) 12974_2018_1105_MOESM5_ESM.docx (13K) GUID:?CBE4A925-F5FE-442A-824F-89D5EA831080 Additional file 6: Table S5. Comparison of levels of phosphorylated proteins between RRMS and SPMS patients in baseline conditions. Levels of phosphorylated proteins in each cell CP-690550 ic50 type in RRMS and SPMS patients. Values represent the mean fluorescence intensity and standard deviation for each group. (DOCX 14?kb) 12974_2018_1105_MOESM6_ESM.docx (15K) GUID:?FB9D2B10-2830-4DA3-B306-F5FCFF8EAD64 Additional file 7: Table S6. Comparison of levels of phosphorylated proteins between RRMS and SPMS patients after in vitro stimulation. Levels of phosphorylated proteins in each cell type in RRMS and SPMS patients. Values represent the mean fluorescence intensity and standard deviation for each group. (DOCX 15?kb) 12974_2018_1105_MOESM7_ESM.docx (15K) GUID:?DB20BFF9-DC64-4A83-84E2-F01E63653B7A Additional file 8: Table S7. Correlation between MS genetic burden and MS risk loci CP-690550 ic50 with levels of phosphorylated proteins in different cell types (baseline). Correlation between baseline levels of phosphorylated proteins and the MSGB (MS genetic burden), MSPBphos (pathway burden of protein phosphorylation ontological family), or MSPBregphos (pathway burden of regulation of protein phosphorylation ontological family) in each cell type. Cor: Spearman coefficient; p: values. Significant correlations are highlighted in bold. (DOCX 21?kb) 12974_2018_1105_MOESM8_ESM.docx (21K) GUID:?6A17B6FD-48DB-442A-B01D-E9DDCF841740 Additional file 9: Desk S8. Relationship between MS hereditary burden and MS risk loci with degrees of phosphorylated proteins after in vitro excitement in various cell types. Relationship between levels of phosphorylated proteins after in vitro stimulation and the MSGB (MS genetic burden), MSPBphos (pathway burden of protein phosphorylation ontological family), or MSPBregphos (pathway burden of regulation of protein phosphorylation ontological family) in each cell type analyzed. Cor: Spearman coefficient; p: values. Significant correlations are highlighted in bold. (DOCX 17?kb) 12974_2018_1105_MOESM9_ESM.docx (17K) GUID:?219BE786-5E91-4BB5-B569-C95C7267D4C0 Additional file 10: Table S9. Correlation between STAT phosphorylation and HLA-E, HLA-ABC, and HLA-DR expression in different cell populations. Correlation between levels of p-STAT1, p-STAT3, p-STAT4, p-STAT5, and p-STAT6 proteins and HLA-E, HLA-ABC, and HLA-DR expression after IFN- or IFN- stimulation. Cor: Spearman coefficient; p: values. Significant correlations are highlighted in bold. (DOCX 15?kb) 12974_2018_1105_MOESM10_ESM.docx (16K) GUID:?F996EEBB-553D-47EB-B286-7B8219503C9D Data Availability StatementPlease contact author for data requests. Abstract Background Multiple sclerosis (MS) is characterized by increased activation of peripheral blood mononuclear cells (PBMCs), linked to perturbations in the phosphorylation of signaling proteins. Methods We developed a phosphoflow cytometry protocol to CP-690550 ic50 assess the levels of 11 phosphorylated nuclear proteins at baseline conditions and after cell activation in distinct PBMC populations from 41 treatment-na?ve relapsing-remitting (RR) MS subjects and 37 healthy controls, and in a second cohort of 9 neglected RRMS individuals and 10 supplementary progressive (SP) MS individuals. Degrees of HLA-ABC, HLA-E, and HLA-DR were assessed also. Phosphorylation degrees of chosen proteins had been also evaluated in mouse splenocytes isolated from myelin oligodendrocyte glycoprotein (MOG)35C55-induced experimental autoimmune encephalomyelitis (EAE). Outcomes Modest variations had been noticed at baseline between settings and individuals, with general lower phosphorylation amounts in cells from individuals. Conversely, a dramatic upsurge in phosphorylated p38MAPK and STAT protein was noticed across all cell.