Background The lipid fraction of rubber ((kunth. poisonous potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSOh, RSOchl+mth and RSOeth and LC50 were found to be (211.70?%, 139.40?%, and 117.41?%, respectively). Conclusions This can be attributed no hazardous linamarin were found in RSO. oil [13], with average range saponification number of (175C250) [14]. The unsaponifiable matter is important to determine the quantity of substances present in the RSO and the quality of RSO. The value of unsaponifiable matter of RSO is 1.83??0.01?% (Table ?(Table1).1). This value is in agreement with the value reported in by [2]. The fatty acid composition of the RSO is shown in (Table ?(Table2).2). The fatty acids of RSO consist from saturated FA (19.12??0.28?%) and unsaturated FA (79.45??0.31?%). The saturated FA are consisting palmitic acid (8 mainly.56??0.07?%) and stearic acidity (10.56??0.02?%), and unsaturated FA are consisting primarily oleic acidity (22.95??0.15?%), linoleic acidity (37.28??0.10?%), and linolenic acidity (19.22??0.21?%). The fatty acidity structure of RSO could be utilized as sign of kind of each Tbx1 fatty acidity [15]. The toxicological substance such as for example linamarin in RSO extracted using hexane was also completed. The primary peaks and their task to functional groups are given in Table ?Table3.3. FTIR spectrum analysis was carried out to determine the presence of such compounds. The carbonyl band occurs as a doublet. Probably FTIR spectrum showed characteristics absorption bands of RSO at wave number (1744?cm-1) for the ester carbonyl functional groups (C?=?O). Peaks at 3009, 2924, 2925 and 2854?cm-1 indicated the CH2 and CH3 scissoring of RSO. The FTIR spectroscopy analysis of RSO indicated presence of sharp peaks at 1463C1413?cm-1 which belong to double 154652-83-2 bond (C?=?C) (Aliphatic). The peaks at 1284C1244?cm-1 of RSO referred to (C-O-C) stretching. The peaks at 1711?cm-1 of RSO referred to the presence of (?COOH) carboxylic acid. FTIR spectrum also showed absorption bands at 722?cm-1 for (C-H) group vibration. FTIR spectrum of RSO did not show any presence of cyanide peak. The result shows that no functional groups that associated with linamarin. Table 3 The main peaks in the FTIR functional groups of RSO Two RSO were studied for cyanide determination. The RSO which was studied in this method was extracted using hexane as a solvent. The commercial cyanide was used as a standard and was compared with RSO which was extracted using hexane. The determination of cyanide exhibited 154652-83-2 no response of the cyanide in RSO and did not 154652-83-2 show any colored comparing with commercial cyanide which observed blue color. The results of current method support the FTIR method that no cyanide observed in this measurement. The commercial cyanide showed high response at 630?nm which is reported at [16]. The colorimetric method based on k?nig reaction showed no response for the detection of cyanide in the RSO. The response of cyanide and RSO are shown in Physique ?Figure22. Physique 2 The response of cyanide (a) and RSO (b). Toxicological evaluation of the RSO was carried out in white male rats by performing an acute toxicity limit test to assess its acute toxicity potential in 3?months feeding study. Three different types of RSO were extracted by using different solvents, such as, hexane (RSOh), chloroform?+?methanol 154652-83-2 (RSOch+mth) and ethanol (RSOeth), in the same extracting condition. Table ?Table44 shows the mortality, color and behavior of the male white rats. Desk 4 Rats toxicological check: six rats The.